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Finally, with the assistance of computers, a series of computational methods such as molecular docking, molecular dynamics simulation, and quantum mechanical methods are used to predict and evaluate the changes in beneficial mutants in terms of structure, free energy, and substrate binding energy [85]
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In phase two, Sprague–Dawley rats (8 weeks old) were randomly assigned to 3 groups and received either purified water, L-citrulline (500 mg/kg/day), or a combination of L-citrulline (500 mg/kg/day) and GSH (50 mg/kg/day) by oral gavage for 3 days
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